Methods
For our research, we frequently employ the following techniques:
- in vitro cell culture experiments with lymphatic endothelial cells and leukocytes
- Fluorescence-Activated Cell Sorting for cell analysis and isolation (ETH Flow Cytometry Core Facility)
- in vivo experiments in mice (models of inflammation and of leukocyte trafficking)
- immunofluorescence / immunohistochemistry
- standard biochemical and molecular biology techniques, such as
qRT-PCR, siRNA technology, gene overexpression, Western blot, ELISA
- transcriptomics analysis, in collaboration with the external pageFGCZcall_made
- confocal and multiphoton intravital microscopy, in collaboration with the ETH SCOPEM